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The Theory and Practice of Lineage Tracing - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618107/
Lineage tracing is a method that delineates all progeny produced by a single cell or a group of cells. The possibility of performing lineage tracing initiated the field of Developmental Biology, and continues to revolutionize Stem Cell Biology. Here, I introduce the principles behind a successful lineage-tracing experiment.
Methods in lineage tracing - ScienceDirect
https://www.sciencedirect.com/science/article/pii/B9780128148419000816
Lineage tracing is a technique used to identify and track cell populations in vivo. Genetic lineage tracing is generally performed by combining an inducible Cre expressed in a cell type of interest with a Cre-activated reporter usually expressing a fluorescent protein.
Scientists Develop New Method to Trace Cell Lineage
https://news.feinberg.northwestern.edu/2020/07/scientists-develop-new-method-to-trace-cell-lineage/
Northwestern Medicine scientists have developed a new method to trace cell lineage, which can help determine the origin of mammalian neuron cell populations from progenitor cells, according to a study published in the journal Developmental Cell.
Lineage Tracing: Cell
https://www.cell.com/abstract/S0092-8674%2812%2900003-7
In lineage tracing, a single cell is marked in such a way that the mark is transmitted to the cell's progeny, resulting in a set of labeled clones. Lineage tracing provides information about the number of progeny of the founder cell, their location, and their differentiation status.
Lineage tracing - PubMed
https://pubmed.ncbi.nlm.nih.gov/22265400/
Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19 (th) century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues.
Lineage tracing meets single-cell omics: opportunities …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307462/
Labelling of cells for prospective lineage tracing can be performed at clonal resolution (such as by delivering complex barcode libraries) or, alternatively, by documenting the collective fate of a population of cells (such as by delivering a common label to the cell population).
Methods for lineage tracing on the organism-wide level
https://pubmed.ncbi.nlm.nih.gov/29175321/
Methods for lineage tracing on the organism-wide level Determining the lineage origin of cell types is a major goal in developmental biology. Furthermore, lineage tracing is a powerful approach for understanding the origin of developmental defects as well as the origin of diseases such as cancer.
Lineage tracing: technology tool for exploring the …
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-020-01941-y
Moreover, a variety of cell transfection methods have emerged, such as calcium phosphate nanotechnology and liposome technology (Fig. 2 b, c) [ 17, 18 ]. However, transfection impairs the normal growth and development of cells and is …
Tracing cell relationships | Nature Methods
https://www.nature.com/articles/s41592-021-01370-6
Lineage tracing methods help unravel complexities of cell state and differentiation programs. Lineage tracing methods allow the study of cell differentiation trajectories while providing a record...
Integrating lineage tracing and single-cell analysis
https://www.nature.com/articles/s41592-020-0802-3
Single-cell genomic profiling and lineage tracing are two categories of powerful tools in developmental biologists’ arsenal. Despite their popularity, their weaknesses are as well known as their...
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