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Multiple neuroanatomical tract-tracing using fluorescent ...
https://pubmed.ncbi.nlm.nih.gov/19617887/#:~:text=Cholera%20toxin%20subunit%20B%20%28CTB%29%20is%20a%20highly,reliably%20studied%20and%20compared%20in%20the%20same%20animal.
A versatile cholera toxin conjugate for neuronal targeting ...
https://pubmed.ncbi.nlm.nih.gov/32355935/
Tracing of neurons plays an essential role in elucidating neural networks in the brain and spinal cord. Cholera toxin B subunit (CTB) is already widely used as a tracer although its use is limited by the need for immunohistochemical detection. A new construct incorporating non-canonical azido amino acids (azido-CTB) offers a novel way to expand the range and flexibility of this …
Cholera Toxin B Subunit Shows Transneuronal Tracing …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671609/
The cholera toxin B subunit (CTB) binds to cellular surfaces via its receptor monosialoanglioside (GM1) . As a conventional bacterial toxin for neuronal tracing, CTB enters the axons or dendrites by active uptake via nerve terminals, or through severed axons .
Tracing of neuronal connections with cholera toxin …
https://pubmed.ncbi.nlm.nih.gov/2458510/
Abstract Subunit B of cholera toxin was used as a tracer substance in the central nervous system after being injected into various brain regions, mainly somatosensory relay structures. The tracer was localized with an immunoperoxidase technique, using monoclonal antibodies raised in mouse hybridomas.
Cholera Toxin B Subunit Shows Transneuronal Tracing …
https://pubmed.ncbi.nlm.nih.gov/26640949/
Cholera toxin B subunit (CTB) has been extensively used in the past for monosynaptic mapping. For decades, it was thought to lack the ability of transneuronal tracing. In order to investigate whether biotin conjugates of CTB (b-CTB) would pass through transneurons in the rat spinal cord, it was injected into the crushed left sciatic nerve.
Retrograde neuronal tracing with cholera toxin B subunit ...
https://pubmed.ncbi.nlm.nih.gov/7868356/
Retrograde neuronal tracing with cholera toxin B subunit: comparison of three different visualization methods Abstract In this report a comparison is made of three different visualization methods of rat cervical motoneurons retrogradely labelled with cholera toxin B subunit (CTb).
Multiple neuroanatomical tract-tracing using fluorescent ...
https://pubmed.ncbi.nlm.nih.gov/19617887/
Multiple neuroanatomical tract-tracing using fluorescent Alexa Fluor conjugates of cholera toxin subunit B in rats. Cholera toxin subunit B (CTB) is a highly sensitive retrograde neuroanatomical tracer. With the new availability of fluorescent Alexa Fluor (AF) conjugates of CTB, multiple neuroanatomical connections can be reliably studied and compared in the same animal.
Alexa Fluor 488-conjugated cholera toxin subunit B ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083145/
Neural tract tracing is used to study neural pathways and evaluate neuronal regeneration following nerve injuries. However, it is not always clear which tracer should be used to yield optimal results. In this study, we examined the use of Alexa Fluor 488-conjugated cholera toxin subunit B (AF488-CTB).
Endocytosis of cholera toxin into neuronal GERL.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC392655/
Cholera toxin is not toxic to neurons at the levels used. These results indicate that GERL is the primary site of endocytosis of presumed complexes of cholera toxin with its plasma membrane receptor (GM1 ganglioside-containing moieties). It is suggested that, in neurons, plasma-membrane bound ligands are taken up primarily into GERL. Full text
Frontiers | A Student’s Guide to Neural Circuit Tracing ...
https://www.frontiersin.org/articles/10.3389/fnins.2019.00897/full
Cholera toxin subunit B (CTb) was introduced as a retrograde tracer in 1977 ( Stoeckel et al., 1977; Vercelli et al., 2000 ). Trojanowski et al. (1981, 1982) then sought to improve the sensitivity of HRP using CTb-HRP conjugates, and found that when the number and detail of labeled neurons was compared, CTb-HRP significantly out-performed free HRP.
Did you choose appropriate tracer for retrograde tracing ...
https://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0205133
Cholera toxin subunit B (CTB) and Fluorogold (FG) are two widely utilized retrograde tracers to assess the number and function of retinal ganglion cells (RGCs). However, the relative advantages and disadvantages of these tracers remain unclear, which may lead to their inappropriate application.
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