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Lineage tracing mediated by cre-recombinase activity
https://pubmed.ncbi.nlm.nih.gov/25501382/
Lineage tracing mediated by cre-recombinase activity J Invest Dermatol. 2015 Jan;135(1):1-4. doi: 10.1038/jid.2014.472. Authors Susanne Vorhagen 1 , Joanna Jackow 2 , Simona Georgina Mohor 3 , Giel Tanghe 4 , Luna Tanrikulu 5 , Claudia Skazik-Vogt 6 , Frederik Tellkamp 7 Affiliations 1 Department of Dermatology, Center for Molecular Medicine ...
Genetic lineage tracing with multiple DNA recombinases: …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212637/
For genetic lineage tracing, inducible Cre recombinase was developed with an attempt to achieve temporal and spatial control of recombination. To facilitate inducible recombination, the human estrogen receptor (ER) is fused to Cre recombinase. Because it binds to heat shock proteins (HSPs), the Cre-ER fusion protein is held in the cytoplasm.
Genetic lineage tracing with multiple DNA recombinases: …
https://pubmed.ncbi.nlm.nih.gov/32213599/
Site-specific recombinases, such as Cre, are a widely used tool for genetic lineage tracing in the fields of developmental biology, neural science, stem cell biology, and regenerative medicine. However, nonspecific cell labeling by some genetic Cre tools remains a …
Application of Cre-loxP recombination for lineage tracing …
https://pubmed.ncbi.nlm.nih.gov/22688702/
Application of Cre-loxP recombination for lineage tracing of adult zebrafish retinal stem cells Abstract The Cre-loxP recombination system is widely used as a genetic tool to achieve conditional gene expression and for lineage tracing. Though extensively used in mice, this technology has only recently been applied to zebrafish.
Lineage Tracing Mediated by Cre-Recombinase Activity
https://www.jidonline.org/article/S0022-202X(15)37063-9/fulltext
In cells in the bulge cell lineage (green cells), the Cre-recombinase mediates the deletion of the loxP site–flanked stop codon in front of the reporter gene and thereby induces reporter gene expression. This permanent genetic modification is passed on to the cell progeny, independent of Cre expression, and facilitates tracing of the cell lineage.
Cre Recombinase Driver Mice Reveal Lineage-Dependent …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371926/
The constitutive BRS3-Cre mice show a reporter signal (Cre-dependent tdTomato) in the adult brain because of lineage tracing in the dentate gyrus, striatal patches, and indusium griseum, in addition to sites previously identified in the inducible BRS3-Cre mice (including hypothalamic and amygdala subregions, and parabrachial nucleus).
Lineage Tracing Mediated by Cre-Recombinase Activity
https://core.ac.uk/download/pdf/82146261.pdf
expression, and facilitates tracing of the cell lineage. In cells that are not in the bulge cell lineage (gray cell), the Cre gene is not expressed and therefore the reporter gene will not be expressed. fe 2. igur lineage tracing example: inducible Cre-recombination via the Tet-Off system. Mannik et al. (2010) used a lineage tracing approach to
Enhancing the precision of genetic lineage tracing using …
https://pubmed.ncbi.nlm.nih.gov/29131159/
The Cre-loxP recombination system is the most widely used technology for in vivo tracing of stem or progenitor cell lineages. The precision of this genetic system largely depends on the specificity of Cre recombinase expression in targeted stem or progenitor cells. However, Cre expression in nontarg …
The Theory and Practice of Lineage Tracing - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618107/
Expression of Cre recombinase is under the control of a cell-type or tissue-specific promoter. This line is then crossed to a reporter line carrying enzymes or fluorescent proteins inserted in a ubiquitously expressed locus such as Rosa26. Reporter expression is prevented by a stop cassette flanked by two loxP sites.
A novel lineage-tracing mouse model for studying early …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9084889/
As a control for our lineage-tracing model, we use CAG-Cre plasmid which drives the expression of a self-deleting Cre recombinase, just as in the MmuPV1-lox-Cre-lox plasmid, but it does not encode any MmuPV1 genes. As performed above, pCAG-Cre was delivered to R26R-lox-STOP-lox-eYFP mice after UV-B irradiation and tail skin scarification.
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