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Neuronal tracing with DiI: decalcification, cryosectioning, …
https://pubmed.ncbi.nlm.nih.gov/2185313/
We describe three modifications of this tracing method. First, while DiL diffuses along neuronal membranes the tissue can be decalcified in EDTA at 37 degrees C. Tracing in decalcified tissue extends the possible application of the DiI technique to the investigation of neuronal tissue enclosed in bony structures.
Neural tract tracing using Di-I: a review and a new method …
https://pubmed.ncbi.nlm.nih.gov/11074091/
The use of Di-I in tract-tracing is briefly reviewed and a novel delayed-fixation approach to neural tract-tracing in the postmortem human adult brain is reported. Using the new approach, fast Di-I, a highly lipophilic fluorescent dye was injected into a particular region or nucleus and labelled tracts were followed for distances of some 20-40 mm.
DiI Labeling - Protocols - Confluence
https://zfin.atlassian.net/wiki/spaces/prot/pages/356155812/DiI+Labeling
1. Mount embryos in 1.2% agar (see Agar Mounting, page 4.5). 2. Use a razor blade to cut flat faces in the agar approximately 1 mm from the embryos on the side to be injected and on the side directly opposite. Figure 11. Agar embedding. 3. Slide the agar containing the embryos until it rests against the edge of the bridged coverslip.
DiI - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/medicine-and-dentistry/dii
2 Protocol 1. Prepare DiI/DMSO stock by mixing ca. 60 mg of DiI paste (one scoop with a 200 μL tip) in 500–1000 μL of DMSO. 2. Prepare embryo medium containing 7% DiI/DMSO stock and 1% ethanol. Warm up to 30 °C. 3. Incubate live embryos in the solution from Step 2 at 30 °C for 10 min. 4. Wash embryos in embryo medium at 28 °C.
Anterograde Tracing Method using DiI to Label Vagal …
https://www.ncbi.nlm.nih.gov/sites/ppmc/articles/PMC1974840/
The mouse is an extremely valuable model for studying vagal development in relation to strain differences, genetic variation, gene manipulations, or pharmacological manipulations. Therefore, a method using 1, 1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine ...
DiD, DiI, DiO and DiR for Labeling Cell Membranes - AAT Bio
https://docs.aatbio.com/products/protocol/22101.pdf
4.1 Slection of DiD, DiO, DiI and DiR’s filter sets are summarized in Table 1. 4.2 For simultaneous detection of multiple dyes, multiband filter sets are available as follows: a) DiI and DiO = Omega XF52, Chroma 51004 b) DiI and DiD = Omega XF92, Chroma 51007 c) DiI, DiO and DiD = Omega XF93, Chroma 61005 5. Flow Cytometry Detection
Lipophilic Tracers—Dil, DiO, DiD, DiA, and DiR - US
https://tools.thermofisher.com/content/sfs/manuals/mp00282.pdf
Sulfonated derivatives of DiI and DiO, and CM-DiI (a thiol-reactive DiI derivative) pro-duce staining that persists after fixation and permeabilization treatments. These tracers are described in instruction manual MP 06999. In addition to neuronal tracing, lipophilic carbocyanines have many other applications in-cluding:
DiD, DiI, DiO, DiR and DiS for Labeling Cell Membranes
https://docs.aatbio.com/products/protocol/22033.pdf
DiI (22035, 22044, 22050, 22052, 22101, 22102, 22103) DiR (22070) DiS (22073, 22076) ... imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of ... Sample Protocol 1. Prepare DiO, DiI DiD, DiS or DiR membrane stain solutions:
Dil Stain (1,1'-Dioctadecyl-3,3,3',3' …
https://www.thermofisher.com/order/catalog/product/D282
Recommendations DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells.
Tracers for Membrane Labeling—Section 14.4 | Thermo …
https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/fluorescent-tracers-of-cell-morphology-and-fluid-flow/tracers-for-membrane-labeling.html
DiI and DiD can be used simultaneously for two-color tracing and as a fluorescence donor–acceptor pair in excited-state energy transfer studies. Combinations of DiO, ... Detailed protocols for DiI labeling and confocal laser-scanning microscopy have been published. DiI has also been used to mark sites of microinjection by coating the outside ...
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