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Identifying Adult Stem Cells Using Cre-Mediated Lineage …
https://pubmed.ncbi.nlm.nih.gov/26840226/
Identifying Adult Stem Cells Using Cre-Mediated Lineage Tracing Lineage-tracing has been used for decades to establish cell fate maps during development. Recently, with the advent of genetic lineage-tracing techniques (employing Cre-lox recombination), it has been possible to permanently mark progenitor/stem cell populations within somatic tissues.
Lineage Tracing Using Cux2-Cre and Cux2-CreERT2 Mice
https://pubmed.ncbi.nlm.nih.gov/25996136/
Lineage Tracing Using Cux2-Cre and Cux2-CreERT2 Mice Using genetic fate-mapping with Cux2-Cre and Cux2-CreERT2 mice we demonstrated that the neocortical ventricular zone (VZ) contains radial glial cells (RGCs) with restricted fate potentials (Franco et al., 2012).
Genetic lineage tracing with multiple DNA recombinases: …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212637/
For genetic lineage tracing, inducible Cre recombinase was developed with an attempt to achieve temporal and spatial control of recombination. To facilitate inducible recombination, the human estrogen receptor (ER) is fused to Cre recombinase. Because it binds to heat shock proteins (HSPs), the Cre-ER fusion protein is held in the cytoplasm.
Lineage Tracing Mediated by Cre-Recombinase Activity
https://www.jidonline.org/article/S0022-202X(15)37063-9/pdf
• Lineage tracing enables us to study the dynamics of proliferation, differentiation, and migration of these cells in an in vivocontext during development, homeostasis, and disease. lIMITaTIONs • detailed characterization of the Cre-recombinase A and the reporter genes, in terms of both activity and specificity, is required to prevent system
The Theory and Practice of Lineage Tracing - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618107/
Lineage tracing is a method that delineates all progeny produced by a single cell or a group of cells. The possibility of performing lineage tracing initiated the field of Developmental Biology, and continues to revolutionize Stem Cell Biology. Here, I introduce the principles behind a successful lineage-tracing experiment.
Lineage Tracing Using Cux2-Cre and Cux2-CreERT2 Mice
https://www.sciencedirect.com/science/article/pii/S0896627315003694
Most neurons of the Cux2-Cre lineage expressed Satb2 ( Franco et al., 2012 ), which is used as a marker for callosal projection neurons in upper and lower layers and for locally projecting neurons in layer 4 ( Alcamo et al., 2008, Arlotta et al., 2005, Britanova et al., 2008 ). We will refer to these neurons as corticocortical projection neurons.
Lineage Tracing - ScienceDirect
https://www.sciencedirect.com/science/article/pii/S0092867412000037
Lineage tracing provides information about the number of progeny of the founder cell, their location, and their differentiation status. Used in classical developmental biology, lineage tracing is increasingly applied to stem cell research and in …
Lineage tracing: technology tool for exploring the …
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-020-01941-y
Lineage tracing is the most widely used technique to track the migration, proliferation, and differentiation of specific cells in vivo. The currently available gene-targeting technologies have been developing for decades to study organogenesis, tissue injury repairing, and tumor progression by tracing the fates of individual cells.
Lineage Tracing: Cell
https://www.cell.com/fulltext/S0092-8674(12)00003-7
Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19 th century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues.
Cre-Lox recombination - Wikipedia
https://en.wikipedia.org/wiki/Cre-Lox_recombination
The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.
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