Lineage Tracing Of Pancreatic Stem Cells And Beta Cell Regeneration

Lineage tracing of pancreatic stem cells and beta cell ...

https://pubmed.ncbi.nlm.nih.gov/22893416/

Postnatal islet regeneration may or may not depend on an influx of new islet cells from adult progenitors. To solve this issue in animal models, genetic lineage tracing has become a crucial research method. This method allows to test the various hypotheses that have been proposed concerning β cell neogenesis and regeneration.

Lineage Tracing of Pancreatic Stem Cells and Beta Cell ...

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Lineage tracing of pancreatic stem cells and beta cell ...

https://www.semanticscholar.org/paper/Lineage-tracing-of-pancreatic-stem-cells-and-beta-Houbracken-Mathijs/8eff85f44a6cf40e3d72f7194e5d4dc3d9b8f651

This method allows to test the various hypotheses that have been proposed concerning β cell neogenesis and regeneration and it is shown that genetic lineage tracing has become a crucial research method for solving this issue in animal models. Restoring a functional β cell mass in diabetes patients by β cell transplantation or stimulation of β cell regeneration are promising …

Lineage Tracing of Pancreatic Stem Cells and Beta Cell ...

https://www.researchgate.net/publication/230671799_Lineage_Tracing_of_Pancreatic_Stem_Cells_and_Beta_Cell_Regeneration

Fred Levine. Because type 1 and type 2 diabetes are characterized by loss of β-cells, β-cell regeneration has garnered great interest as an approach to diabetes therapy. Here, we developed a …

Lineage tracing and resulting phenotype of haemopoietic ...

https://pubmed.ncbi.nlm.nih.gov/20585934/

Aims: Transplantation of bone marrow-derived haemopoietic stem cells following streptozotocin (STZ) treatment to induce pancreatic beta cell loss in mice causes the partial regeneration of beta cell mass, with many haemopoietic cells demonstrating endothelial cell markers. This study used genetically tagged haemopoietic lineage-derived cells to determine how endogenous …

Pancreatic beta-cells: from generation to regeneration - …

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2967617/

In addition, using a novel DNA analog-based lineage tracing approach, it was found that only beta-cells contribute to the beta-cell regeneration occurring under normal physiological conditions, following 50% PPX or treatment with the GLP-1 agonist Exendin-4, and during pregnancy, underscoring the importance of replication to sustain the beta ...

Lineage tracing and resulting phenotype of …

https://link.springer.com/content/pdf/10.1007/s00125-010-1835-4.pdf

to induce pancreatic beta cell loss in mice causes the partial regeneration of beta cell mass, with many haemopoietic cells demonstrating endothelial cell markers. This study used genetically tagged haemopoietic lineage-derived cells to determine how endogenous cells are mobilised following beta cell loss and subsequent replacement.

Pancreatic beta cell neogenesis: Debates and updates: …

https://www.cell.com/cell-metabolism/fulltext/S1550-4131(21)00485-X

Finding endogenous, renewable sources for insulin-producing beta cells in the adult pancreas is one of the holy grails of stem cell research and regenerative medicine. Through lineage tracing and scRNA-seq approaches, Gribben et al. have recently reported that Ngn3-expressing ductal cells could serve as progenitors for new beta cells in the adult pancreas.

Pancreatic β cell regeneration: to β or not to β ...

https://www.sciencedirect.com/science/article/pii/S2468867319301592

A population of immature β cells identified by the presence of insulin expression, but absence of the maturity marker urocortin3 (Ucn3) were found at the periphery of the islet, and are thought to be in a transition state between mature α cells and β cells [76 ••]. Lineage tracing confirmed that these cells once expressed glucagon.

Lineage Reprogramming: A Promising Road for …

https://www.sciencedirect.com/science/article/pii/S1043276016000151

Subsequently, a genetic lineage tracing approach helped in confirming that the β cells originated from acinar cells both in cultured adult pancreatic exocrine cells and in diabetic mice treated with cytokines, and the reprogramming of acinar cells was dependent on the activation of Ngn3. In summary, acini-to-β cell conversion can be completed ...