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Monosynaptic tracing: a step-by-step protocol - PubMed
https://pubmed.ncbi.nlm.nih.gov/31408693/
Monosynaptic tracing: a step-by-step protocol
https://www.sciencedirect.com/science/article/pii/S0891061819300560
"Monosynaptic tracing" refers to the use of a deletion-mutant neurotropic virus that is complemented in vivo selectively within a targeted neuronal population of interest, so that the virus can replicate within them and spread to other neurons that are in direct synaptic contact ( Wickersham et al., 2007a ).
Monosynaptic Circuit Tracing with Glycoprotein-Deleted …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4469731/
Monosynaptic rabies tracing of inputs to Cre-expressing starter cells. Monosynaptic tracing with G-deleted rabies (RV dG) involves expression of the EnvA receptor, TVA, and rabies glycoprotein (G) in starter cells.
Monosynaptic Circuit Tracing with Glycoprotein-Deleted …
https://pubmed.ncbi.nlm.nih.gov/26085623/
Monosynaptic Circuit Tracing with Glycoprotein-Deleted Rabies Viruses J Neurosci. 2015 Jun 17;35(24):8979-85. doi: 10.1523/JNEUROSCI.0409-15.2015. Authors Edward M Callaway 1 , Liqun Luo 2 Affiliations 1 Systems Neurobiology Labs, The Salk Institute for ...
Monosynaptic tracing: a step-by-step protocol
https://www.sciencedirect.com/science/article/abs/pii/S0891061819300560
Monosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons.
Monosynaptic circuit tracing in vivo through Cre …
https://pubmed.ncbi.nlm.nih.gov/21115815/
We describe a powerful system for revealing the direct monosynaptic inputs to specific cell types in Cre-expressing transgenic mice through the use of Cre-dependent helper virus and a modified rabies virus. ... Monosynaptic circuit tracing in vivo through Cre-dependent targeting and complementation of modified rabies virus Proc Natl Acad Sci U ...
Monosynaptic tracing maps brain-wide afferent …
https://elifesciences.org/articles/49291
Here, we employ a modified rabies virus-based monosynaptically-restricted trans-synaptic retrograde tracing strategy to elucidate a map of neuronal synaptic inputs to OPCs in the corpus callosum (CC), secondary motor cortex (MOs), and …
Monosynaptic restriction of transsynaptic tracing from …
https://pubmed.ncbi.nlm.nih.gov/17329205/
Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons Abstract There has never been a wholesale way of identifying neurons that are monosynaptically connected either to some other cell group or, especially, to a single cell.
Addgene: AAV for Neuronal Tracing
https://www.addgene.org/viral-service/aav-prep/tracing/
AAV Vectors for Monosynaptic Neuronal Tracing Introduction to Rabies Virus-based Monosynaptic Tracing The core idea of the system is to infect the cell population of interest with a deletion-mutant tracing virus that lacks the ability to infect neighboring cells.
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