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Transsynaptic Tracing from Peripheral Targets with …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692604/
Transsynaptic tracing has become a powerful tool used to analyze central efferents that regulate peripheral targets through multi-synaptic circuits. This approach has been most extensively used in the rodent brain by utilizing the swine pathogen pseudorabies virus (PRV), especially the attenuated strain PRV-Bartha first described in 1961 12.
Pseudorabies virus tracing of neural pathways between …
https://pubmed.ncbi.nlm.nih.gov/10713575/
The transneuronal tracer, pseudorabies virus (PRV), was used to identify pathways from the uterine cervix which may be involved in induction of analgesia and abbreviation of estrus by vaginocervical stimulation.
[Studies on neuronal tracing with pseudorabies virus]
https://pubmed.ncbi.nlm.nih.gov/25118391/
[Studies on neuronal tracing with pseudorabies virus] Abstract With its abilities of trans-synaptic tracing and self-replication and wide host range, pseudorabies virus (PRV) has been applied in the field of neuroanatomy since the 1970s. Four decades of PRV application have made many advances in researches on neuronal tracing with PRV.
Dual-Pseudorabies Viral Tracing for Spinal Tyrosine …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742299/
several studies have completed extensive tracing experiments of the lut network in an effort to illustrate the complexity of this system.13–15pseudorabies virus (prv) is transmitted from an infected neuron to other neurons through their synaptic connections, thus defining specific pathways involved in neuron-neuron signaling, including spinal …
Pseudorabies virus as a transneuronal tract tracing tool: …
https://www.ncbi.nlm.nih.gov/pubmed/8542383
Pseudorabies virus as a transneuronal tract tracing tool: specificity and applications to the sympathetic nervous system. ... Because of technical shortcomings, neuroanatomical tract tracing methods have been limited in their ability to examine functional pathways. This has been particularly true of the study of the sympathetic nervous system.
Transneuronal Circuit Analysis with Pseudorabies Viruses
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4165084/
( C) A model of virion assembly postulated for pseudorabies virus is illustrated. Assembly of virions is a multistep process that leads to assembly of mature virions in the cell soma. Virions traffic through the soma and dendrites of infected cells and are released in the vicinity of synaptic contacts. Adapted with permission from Card (1998).
Pseudorabies Virus - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/medicine-and-dentistry/pseudorabies-virus
Pseudorabies virus (PRV) is a neurotropic herpesvirus that is commonly used to determine organization of neurological pathways. Viral particles are taken up by axon terminals and transported retrogradely to the cell body, where they replicate and infect connecting neurons across synapses.
Transneuronal circuit analysis with pseudorabies viruses
https://pubmed.ncbi.nlm.nih.gov/18428451/
The method exploits the propensity of neurotropic viruses to invade neurons and then produce infectious progeny that cross synapses to infect other neurons within a circuit. The protocols and commentaries included in this unit focus upon the use of the swine alpha herpesvirus known as pseudorabies virus (PRV) for polysynaptic analysis.
Long-term Cre-mediated retrograde tagging of neurons …
https://pubmed.ncbi.nlm.nih.gov/25232307/
Long-term Cre-mediated retrograde tagging of neurons using a novel recombinant pseudorabies virus Brain regions contain diverse populations of neurons that project to different long-range targets. The study of these subpopulations in circuit function and behavior requires a toolkit to characterize and manipulate their activity in vivo.
Viral neuronal tracing - Wikipedia
https://en.wikipedia.org/wiki/Viral_Neuronal_Tracing
A PC12 neuron infected with pseudorabies virus PRV GS443 (labeled in green). The green dots moving away from the cell body demonstrates anterograde transport. Viral neuronal tracing is the use of a virus to trace neural pathways, providing a self-replicating tracer.
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