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Retrograde Tracing - labome.com
https://www.labome.com/method/Retrograde-Tracing.html
Retrograde tracing is a powerful tool to understand the neuronal circuit and structure/function of brain communication networks in a broad range of animals including rodents and primates. A large number of novel retrograde neuronal tracers based on conventional approaches (such as dyes and fluorescent molecules) are being explored by neuroanatomists …
Retrograde tracing - Wikipedia
https://en.wikipedia.org/wiki/Retrograde_tracing
BAcTrace, a tool for retrograde tracing of neuronal ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610425/
Here we present BAcTrace (Botulinum Activated Tracer), a genetically encoded, retro-grade, transsynaptic labelling system. BAcTrace is based on C. botulinumneurotoxin A, Botox, which we have engineered to travel retrogradely between neurons to activate an otherwise silent transcription factor.
Retrograde neuronal tracing with cholera toxin B subunit ...
https://pubmed.ncbi.nlm.nih.gov/7868356/
Retrograde neuronal tracing with cholera toxin B subunit: comparison of three different visualization methods Abstract In this report a comparison is made of three different visualization methods of rat cervical motoneurons retrogradely labelled with cholera toxin B subunit (CTb).
BAcTrace, a tool for retrograde tracing of neuronal ...
https://www.nature.com/articles/s41592-020-00989-1
Here we present BAcTrace (Botulinum-Activated Tracer), a genetically encoded, retrograde, transsynaptic labeling system. BAcTrace is based on Clostridium botulinum neurotoxin A, Botox, which we...
Retrograde axonal tracing with fluorescent markers
https://pubmed.ncbi.nlm.nih.gov/18428673/
The growth of fluorescence imaging technology and the development of sensitive fluorescent retrograde tracers has provided many new approaches for analyzing neuronal circuits. Fluorescent markers provide unparalleled opportunity for combining axonal tract tracing with techniques such as immunohistochemistry or physiological recording. This unit describes the …
Neuronal tracing - NEUROANATOMY
https://neuroanatomy.org/2003/002_005.pdf
Retrograde axonal transport is used by certain viruses (e.g., herpes and pseudorabies virus) to spread from one neuron to the next in a chain of neurons (transneuronal transfer). It is also the method whereby toxins (e.g., tetanus) are transported from the periphery into the central nervous system. This has led to the idea that viral tracing may be an
Using AAV for neuronal tracing - Addgene
https://blog.addgene.org/using-aav-for-neuronal-tracing
Anterograde tracing outlines neurons from their cell bodies to the terminals of their axons; while retrograde tracing outlines neurons in the opposite direction, from the terminals of their axons to their cell bodies. Anterograde and retrograde tracing take advantage of existing transport pathways in neurons.
Frontiers | A Student’s Guide to Neural Circuit Tracing ...
https://www.frontiersin.org/articles/10.3389/fnins.2019.00897/full
Tracers are categorized as anterograde or retrograde based on their direction of travel within neurons. Anterograde tracers (green) are taken up by neuronal cell bodies at the injection site and travel down the axon to terminal processes. Retrograde tracers (blue) are taken up by terminals and travel back to the cell body.
Viral neuronal tracing - Wikipedia
https://en.wikipedia.org/wiki/Viral_Neuronal_Tracing
Retrograde tracing is the use of a tracer that moves from synapse to soma. Retrograde transport uses a protein called dynein to move viruses along the axon in the retrograde direction. It is important to note that different tracers show characteristic affinities for dynein and kinesin, and so will spread at different rates. Dual transport
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