Interested in racing? We have collected a lot of interesting things about Retrograde Tracing Ctb. Follow the links and you will find all the information you need about Retrograde Tracing Ctb.
Retrograde Tracing - labome.com
https://www.labome.com/method/Retrograde-Tracing.html
Retrograde neuronal tracing with cholera toxin B subunit …
https://pubmed.ncbi.nlm.nih.gov/7868356/
Abstract In this report a comparison is made of three different visualization methods of rat cervical motoneurons retrogradely labelled with cholera toxin B subunit (CTb). CTb conjugates such as CTb-HRP and CTb-FITC or CTb-TRITC, which can be visualized after histochemical detection and by fluorescence microscopy, respectively.
Retrograde neuronal tracing with cholera toxin B subunit: …
https://link.springer.com/article/10.1007/BF00162931
CTb is a very sensitive retrograde neuro-anatomical tracer which can be detected either by immunochemical methods, or by the use of CTb conjugates such as CTb-HRP and CTb-FITC or CTb-TRITC, which can be visualized after histochemical detection and by fluorescence microscopy, respectively. The following results were obtained.
Multiple neuroanatomical tract-tracing using fluorescent …
https://pubmed.ncbi.nlm.nih.gov/19617887/
Cholera toxin subunit B (CTB) is a highly sensitive retrograde neuroanatomical tracer. With the new availability of fluorescent Alexa Fluor (AF) conjugates of CTB, multiple neuroanatomical connections can be reliably studied and compared in the same animal.
Did you choose appropriate tracer for retrograde tracing …
https://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0205133
Cholera toxin subunit B (CTB) and Fluorogold (FG) are retrograde tracers widely used in many laboratories [ 27 – 33 ]. CTB is a nontoxic subunit of the cholera toxin protein complex secreted by Vibrio cholerae that binds to cellular surfaces via the pentasaccharide chain of monosialotetrahexosylganglioside [ 34 ].
Cholera Toxin Subunit B (Recombinant), Alexa Fluor™ …
https://www.thermofisher.com/order/catalog/product/C22841
Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside G M1, making it a powerful tool for retrograde labeling of neurons. This tracer has been used in a variety of applications, including tracing of rat forebrain afferents, projections of the parabrachial region, and neurons of the urinary bladder wall.
Multiple neuroanatomical tract-tracing using ... - Nature …
https://www.nature.com/articles/nprot.2009.93
Cholera toxin subunit B (CTB) is a highly sensitive retrograde neuroanatomical tracer. With the new availability of fluorescent Alexa Fluor (AF) conjugates of CTB, multiple neuroanatomical...
Cholera Toxin B Subunit Shows Transneuronal Tracing …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671609/
As a conventional bacterial toxin for neuronal tracing, CTB enters the axons or dendrites by active uptake via nerve terminals, or through severed axons [ 12 ]. Biotin conjugates of CTB (b-CTB) may greatly enhance the uptake, transport and sensitivity of CTB [ 13 ]. It is possible that b-CTB may enhance uptake but inhibit transneuronal labeling.
A Student’s Guide to Neural Circuit Tracing - Frontiers
https://www.frontiersin.org/articles/10.3389/fnins.2019.00897/full
Cholera toxin subunit B (CTb) was introduced as a retrograde tracer in 1977 ( Stoeckel et al., 1977; Vercelli et al., 2000 ). Trojanowski et al. (1981, 1982) then sought to improve the sensitivity of HRP using CTb-HRP conjugates, and found that when the number and detail of labeled neurons was compared, CTb-HRP significantly out-performed free HRP.
CTB retrograde tracing in the mice. (A) The injection sites …
https://www.researchgate.net/figure/CTB-retrograde-tracing-in-the-mice-A-The-injection-sites-of-mouse-020713-AI-CTB-555_fig3_268229763
A retrograde neural tracer, cholera toxin B subunit (CTB) coupled to green fluorescent dye, was injected into the lateral orbitofrontal cortex (LO) of Dbx1 Cre :Ai14 mice (Fig. 6a).
Got enough information about Retrograde Tracing Ctb?
We hope that the information collected by our experts has provided answers to all your questions. Now let's race!
