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Transsynaptic virus tracing from host brain to subretinal ...
https://pubmed.ncbi.nlm.nih.gov/15654853/
To tackle this difficult task, a little-known but well-established CNS method was chosen: trans-synaptic pseudorabies virus (PRV) tracing. Sheets of E19 rat retina with or without retinal pigment epithelium (RPE) were transplanted to the subretinal space in 33 Royal College of Surgeons (RCS) and transgenic s334ter-5 rats with retinal ...
Transsynaptic Tracing from Peripheral Targets with ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692604/
Transsynaptic tracing has become a powerful tool used to analyze central efferents that regulate peripheral targets through multi-synaptic circuits. This approach has been most extensively used in the rodent brain by utilizing the swine pathogen pseudorabies virus (PRV), especially the attenuated strain PRV-Bartha first described in 1961 12 .
Transsynaptic Tracing with Vesicular Stomatitis Virus ...
https://www.ncbi.nlm.nih.gov/sites/ppmc/articles/PMC3711516/
The use of neurotropic viruses as transsynaptic tracers was first described in the 1960s, but only recently have such viruses gained popularity as a method for labeling neural circuits. The development of retrograde monosynaptic tracing vectors has enabled ...
Transsynaptic Tracing from Peripheral Targets with ...
https://pubmed.ncbi.nlm.nih.gov/26436639/
Transsynaptic tracing has become a powerful tool used to analyze central efferents that regulate peripheral targets through multi-synaptic circuits. This approach has been most extensively used in the brain by utilizing the swine pathogen pseudorabies …
Anterograde or Retrograde Transsynaptic Circuit Tracing in ...
https://pubmed.ncbi.nlm.nih.gov/26729030/
In order to explore the use of such viruses in an expanded host range, we tested the transsynaptic tracing ability of recombinant vesicular stomatitis virus (rVSV) vectors in a variety of organisms. Successful infection and gene expression were achieved in a wide range of organisms, including vertebrate and invertebrate model organisms.
Characterization of transsynaptic tracing with central ...
https://pubmed.ncbi.nlm.nih.gov/10446330/
Abstract. Although transsynaptic tracing with peripheral injection of pseudorabies virus (PRV) has been extensively characterized, several methodological issues related to central application of this tracer have not been addressed. In the present study, we addressed the following three issues by using microinjection of a cocktail containing PRV ...
Anterograde or Retrograde Transsynaptic Circuit Tracing in ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4776322/
Moreover, rVSV enabled transsynaptic tracing of neural circuitry in predictable directions dictated by the viral envelope glycoprotein (G), derived from either VSV or rabies virus (RABV). Anterograde and retrograde labeling, from initial infection and/or viral replication and transmission, was observed in Old and New World monkeys, seahorses ...
Neurotropic virus tracing suggests a membranous‐coating ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7162419/
Moreover, studies utilizing a variety of tracing molecules have shown significant transsynaptic labeling after intracellular injection, indicating that interneuronal transfer indeed occurs at the synaptic junctions (Grafstein,1971; Davis and McKinnon1982; Trojanowski and Schmidt,1984; Luo and Dessem,1996). However, the transsynaptic process is ...
Viral neuronal tracing - Wikipedia
https://en.wikipedia.org/wiki/Viral_Neuronal_Tracing
Viral neuronal tracing is the use of a virus to trace neural pathways, providing a self-replicating tracer. Viruses have the advantage of self replication over molecular tracers, but can also spread too quickly and cause degradation of neural tissue. Viruses which can infect the nervous system, called neurotropic viruses, spread through ...
Anterograde or retrograde transsynaptic labeling of CNS ...
https://pubmed.ncbi.nlm.nih.gov/21825165/
Here we describe the use of vesicular stomatitis virus (VSV) for tracing neuronal connections in vivo. We made VSV vectors that used glycoprotein (G) genes from several other … Anterograde or retrograde transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors Proc Natl Acad Sci U S A. 2011 Sep 13;108 (37 ...
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