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Application of Cre-loxP recombination for lineage tracing …
https://pubmed.ncbi.nlm.nih.gov/22688702/
The Cre-loxP recombination system is widely used as a genetic tool to achieve conditional gene expression and for lineage tracing. Though extensively used in mice, this technology has only recently been applied to zebrafish. Here we describe Cre-loxP methodology for conditional expression of transgenes in zebrafish and their use in lineage ...
How does Cre/loxP recombination work? - InVivo Biosystems
https://invivobiosystems.com/view-from-the-bench/how-does-cre-loxp-recombination-work/
It also provides additional tools for genetic lineage tracing. As more and more of these Cre/loxP lines are made, zebrafish researchers will quickly achieve new genetic discoveries matching the advances this system has provided researchers using the mouse model for over two decades. ... How InVivo uses Cre/loxP recombination. Although Cre-lox ...
Enhancing the precision of genetic lineage tracing using …
https://pubmed.ncbi.nlm.nih.gov/29131159/
However, Cre expression in nontargeted cell types can complicate the interpretation of lineage-tracing studies and has caused controversy in many previous studies. Here we describe a new genetic lineage tracing system that incorporates the Dre-rox recombination system to enhance the precision of conventional Cre-loxP-mediated lineage …
Cre-Lox recombination - Wikipedia
https://en.wikipedia.org/wiki/Cre-Lox_recombination
Application of Cre-loxP Recombination for Lineage …
https://link.springer.com/protocol/10.1007/978-1-61779-848-1_8
The Cre-loxP recombination system is widely used as a genetic tool to achieve conditional gene expression and for lineage tracing. Though extensively used in mice, this technology has only recently been applied to zebrafish.
Genetic lineage tracing with multiple DNA recombinases: …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212637/
During the design of a genetic lineage-tracing experiment, we sometimes encounter difficulties when the definition of a specific cell type relies on the expression of both positive and negative markers (e.g. A + B −). The Cre-loxP strategy labels all A + cells, including the “wanted” cell type (e.g. A + B − cells) and “unwanted ...
Enhancing the precision of genetic lineage tracing using …
https://www.nature.com/articles/nm.4437/
Abstract. The Cre– loxP recombination system is the most widely used technology for in vivo tracing of stem or progenitor cell lineages. The precision of …
Lineage Tracing: Cell
https://www.cell.com/fulltext/S0092-8674(12)00003-7
Genetic lineage tracing in mice is usually performed using the Cre-loxP system (Figure 2B). Cre recombinase is expressed under the control of a tissue- or cell-specific promoter in one mouse line. That line is crossed with a second mouse line in which a reporter is flanked by a loxP-STOP-loxP (“floxed” STOP) sequence.
Triple-cell lineage tracing by a dual reporter on a single …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6970925/
Genetic lineage tracing mediated by DNA site-specific recombination systems has been widely used for cell lineage and fate studies . Similar to the Cre-loxP system, multiple site-specific recombination systems, such as Flpe-Frt, Dre-rox, and Nigri-nox, have been developed and used for cell fate tracing studies (17,– 21). Two types of reporter ...
lineage tracing by Cre - LoxP system（字幕版）_哔哩哔 …
https://www.bilibili.com/video/BV1J5411t73d/
lineage tracing by Cre - LoxP system（字幕版）. 1019播放 · 总弹幕数0 2020-04-09 23:59:09. lineage tracing by Cre - LoxP system（字幕版）. 关注.
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